biolockj.module.classifier.r16s

Class QiimeDeNovoClassifier

java.lang.Object

biolockj.module.BioModuleImpl

biolockj.module.ScriptModuleImpl

biolockj.module.SeqModuleImpl

biolockj.module.classifier.ClassifierModuleImpl

biolockj.module.implicit.qiime.QiimeClassifier

biolockj.module.classifier.r16s.QiimeDeNovoClassifier

All Implemented Interfaces:

BioModule, ClassifierModule, DatabaseModule, ScriptModule, SeqModule, Comparable<BioModule>

public class QiimeDeNovoClassifier
extends QiimeClassifier

This module runs the QIIME pick_de_novo_otus.py script on FastA sequence files in a single script so it is important to allocate sufficient job resources if running in a clustered environment.

GUI Module Name

QIIME de novo Classifier

Field Summary

Fields

Modifier and Type	Field and Description
static String	PICK_OTU_SCRIPT De novo OTU picking script: "pick_de_novo_otus.py"

Fields inherited from class biolockj.module.implicit.qiime.QiimeClassifier

ALPHA_DIV_NULL_VALUE, ALPHA_DIVERSITY_TABLE, COMBINED_FNA, EXE_VSEARCH, EXE_VSEARCH_PARAMS, OTU_SUMMARY_FILE, OTU_TABLE, QIIME_PARAMS, QIIME_PYNAST_ALIGN_DB, QIIME_REF_SEQ_DB, QIIME_REMOVE_CHIMERAS, QIIME_TAXA_DB, REP_SET, SCRIPT_ADD_ALPHA_DIVERSITY, SCRIPT_ADD_LABELS, SCRIPT_CALC_ALPHA_DIVERSITY, SCRIPT_FILTER_OTUS, SCRIPT_PRINT_CONFIG, SCRIPT_SUMMARIZE_BIOM, SCRIPT_SUMMARIZE_TAXA, SUMMARIZE_TAXA_SUPPRESS_BIOM

Fields inherited from class biolockj.module.BioModuleImpl

GZIP_EXT, LOG_EXT, PDF_EXT, RETURN, SH_EXT, TAB_DELIM, TSV_EXT, TXT_EXT

Fields inherited from interface biolockj.module.ScriptModule

SCRIPT_BATCH_SIZE, SCRIPT_DEFAULT_HEADER, SCRIPT_NUM_THREADS, SCRIPT_PERMISSIONS, SCRIPT_TIMEOUT

Fields inherited from interface biolockj.module.BioModule

MAIN_SCRIPT_PREFIX, OUTPUT_DIR, TEMP_DIR

Constructor Summary

Constructors

Constructor and Description
QiimeDeNovoClassifier()

Method Summary

Modifier and Type	Method and Description
List<List<String>>	buildScript(List<File> files) Return bash script lines to pick de novo OTUs by calling QiimeClassifier getPickOtuLines() method.
void	checkDependencies() Call QiimeClassifier checkOtuPickingDependencies() method to verify OTU picking script parameters.
protected String	copyTempOtuTableToOutputDir() The method returns 1 bash script line that will copy the batch "otu_table.biom" from the batchDir to the output directory.

Methods inherited from class biolockj.module.implicit.qiime.QiimeClassifier

buildQiimeDockerConfigLines, buildScriptForPairedReads, cleanUp, getClassifierExe, getClassifierParams, getDB, getDockerDB, getInputFileDir, getInputFiles, getParams, getPickOtuLines, getPostRequisiteModules, getPreRequisiteModules, getSummary, getVsearchParams, getWorkerScriptFunctions, isValidInputModule, validateFileNameUnique

Methods inherited from class biolockj.module.classifier.ClassifierModuleImpl

getClassifierType, validateModuleOrder

Methods inherited from class biolockj.module.SeqModuleImpl

getSeqFiles

Methods inherited from class biolockj.module.ScriptModuleImpl

executeTask, getJobParams, getMainScript, getRuntimeParams, getScriptDir, getScriptErrors, getTimeout, hasScripts

Methods inherited from class biolockj.module.BioModuleImpl

cacheInputFiles, compareTo, equals, findModuleInputFiles, getFileCache, getID, getModuleDir, getOutputDir, getTempDir, init, toString

Methods inherited from class java.lang.Object

clone, finalize, getClass, hashCode, notify, notifyAll, wait, wait, wait

Methods inherited from interface biolockj.module.SeqModule

getSeqFiles

Methods inherited from interface biolockj.module.ScriptModule

getJobParams, getMainScript, getScriptDir, getScriptErrors, getTimeout

Methods inherited from interface biolockj.module.BioModule

executeTask, getID, getModuleDir, getOutputDir, getTempDir, init

Field Detail

PICK_OTU_SCRIPT

public static final String PICK_OTU_SCRIPT

De novo OTU picking script: "pick_de_novo_otus.py"

See Also:

Constant Field Values

Constructor Detail

QiimeDeNovoClassifier

public QiimeDeNovoClassifier()

Method Detail

buildScript

public List<List<String>> buildScript(List<File> files)
                               throws Exception

Return bash script lines to pick de novo OTUs by calling QiimeClassifier getPickOtuLines() method. If property "qiime.removeChimeras" = "Y", use vsearch to identify chimeras and call "filter_otus_from_otu_table.py" to remove them from "otu_table.biom"

Specified by:

buildScript in interface ScriptModule

Overrides:

buildScript in class QiimeClassifier

Parameters:

files - Files in the input directory that contain only forward reads

Returns:

Nested list of bash scripts lines

Throws:

Exception - if unable to generate script lines

checkDependencies

public void checkDependencies()
                       throws Exception

Call QiimeClassifier checkOtuPickingDependencies() method to verify OTU picking script parameters. If not in Docker mode and property "qiime.removeChimeras" = "Y", verify "exe.vsearchParams".

Specified by:

checkDependencies in interface BioModule

Overrides:

checkDependencies in class QiimeClassifier

Throws:

Exception - thrown if missing or invalid dependencies are found

copyTempOtuTableToOutputDir

protected String copyTempOtuTableToOutputDir()

The method returns 1 bash script line that will copy the batch "otu_table.biom" from the batchDir to the output directory.

Returns:

Bash script line to copy table to